DYSTROPHIN; MS X-100UG

Application

Anti-Dystrophin Antibody, clone 1808 detects level of Dystrophin & has been published & validated for use in WB, IH.

Western blot

Immunohistochemistry

Optimal working dilutions must be determined by the end user.

Immunogen

Peripheral membrane proteins extracted from Torpedo postsynaptic membranes.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid in 0.02 M phosphate buffer (pH 7.6), 250 mM NaCl with 0.1% sodium azide.

Format: Purified

Specificity

By Western blot MAB1645 recognizes a single protein having a relative molecular weight of 300 kD. Reacts to mammalian skeletal muscle dystrophin. Shows no staining of mdx mouse muscle. Labels normal human muscle but not Duchenne muscle.

STAINING PATTERN:

On cryostat sections of normal mouse diaphragm MAB1645 gives strong immunofluorescence staining of the entire sarcolemma, with particularly strong staining of neuromuscular junctions.

SPECIES REACTIVITY:

Labels skeletal muscle from rat, chicken and Xenopus laevis. Labels cardiac muscle from rat and normal, but not mdx, mouse. Also labels smooth muscle from chicken gizzard.

Storage and Stability

Maintain at -20°C for up to 12 months in convenient aliquots. Avoid repeated freeze/thaw cycles.